human circrna array arraystar v1.0  (Arraystar inc)

Journal: Journal of Cellular and Molecular Medicine

Article Title: CircFOXP1/FOXP1 promotes osteogenic differentiation in adipose‐derived mesenchymal stem cells and bone regeneration in osteoporosis via miR‐33a‐5p

doi: 10.1111/jcmm.15792

Figure Lengend Snippet: CircRNA expression profiles in OP patients. A, Cluster heat map for top 40 differentially expressed circRNAs (20 up‐regulated and 20 down‐regulated) in OP and non‐OP bone tissues. Red colour represents high expression level, green colour represents low expression level. B and C, Relative expression for six most up‐regulated (B) and six most down‐regulated (C) circRNAs validated by qRT‐PCR. D, Relative expression of circFOXP1 in OP (n = 20) and non‐OP (n = 20) bone tissues determined by qRT‐PCR. E, FOXP1 mRNA expression level in OP (n = 20) and non‐OP (n = 20) bone tissues. F, CircFOXP1 is positively correlated with FOXP1 mRNA in OP tissues. G and H, FOXP1 mRNA (G) and protein (H) levels determined by qRT‐PCR and Western blotting in hASCs with circFOXP1 overexpression or knockdown. All experiments were performed at least three times. Untreated hASCs were used as negative control (NC). ** P < 0.01 vs NC group. OP, osteoporosis; hASCs, human adipose‐derived stem cells

Article Snippet: Labelled cRNAs were hybridized onto the Arraystar Human circRNA Array V1.0.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Over Expression, Knockdown, Negative Control, Derivative Assay